Obtaining the essential oil from the indian carnation (Syzygium Aromaticum) and non-clinical safety assessment using adult zebrafish (Danio Rerio)
DOI:
https://doi.org/10.5281/zenodo.4716803Keywords:
Essential oil. Cloves. Non-clinical safety. Locomotor activity. zebrafish.Abstract
Essential oils are odorous substances, found by various parts of plants. Locomotor activity evaluates drugs that cause locomotor impairment or not. The objective of this work was to obtain the essential oil of cloves and perform a non-clinical safety assessment using zebrafish. The EO was obtained through hydrodistillation. For the open field test, zebrafish received doses of OE (4, 20 and 40 mg / kg); DMSO 3% and DZP 40mg / kg. After 30 min, the animals were added in Petri dishes, and locomotor activity was analyzed by counting the number of CL (5 min). For toxicity assessment, the animals received the same treatments and after 96 h of analysis, the values obtained with the number of ZFa killed were subjected to statistical analysis to estimate DL to kill 50%. The OE had a high eugenol composition and low eugenyl acetate and ß-karyophylene levels. In addition, it changed the zebrafish locomotor system and is considered safe, as it was not toxic until 96 hours of analysis.
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